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Biology
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SNARE蛋白介导的膜融合酶的细胞融合含量的分析
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JoVE Journal
Biology
Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
Please note that all translations are automatically generated.
Click here for the English version.
SNARE蛋白介导的膜融合酶的细胞融合含量的分析
DOI:
10.3791/4378-v
•
09:19 min
•
October 19, 2012
•
Nazarul Hasan
,
David Humphrey
,
Krista Riggs
,
Chuan Hu
1
Department of Biochemistry and Molecular Biology
,
University of Louisville School of Medicine
Chapters
00:05
Title
01:19
Cell Surface SNARE Expression Analysis
03:29
Enzymatic Cell Fusion Assay
06:55
SNARE-mediated Interactions between SNAP-25/Syntaxin1/Syntaxin4 and VAMPs
08:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们已经开发出一种量化SNARE介导膜融合事件由激活的β-半乳糖苷酶的表达的细胞融合实验。
Tags
SNARE-mediated Membrane Fusion
Enzymatic Cell Fusion Assay
SNARE Proteins
V-SNAREs
T-SNAREs
Intracellular Vesicle Fusion
Reconstitution Assays
Cell Fusion Assay
Flipped SNARE Proteins
Microscopic Analysis
Quantification Of Cell Fusion Events
β-galactosidase
Tet-Off Gene Expression System
Tetracycline-controlled Transactivator (tTA)
Reporter Plasmid
LacZ Gene
Tetracycline-response Element (TRE-LacZ)
COS-7 Cells
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