Journal
/
Neuroscience
/
基于miRNA的质粒在发展中的神经管和表型的评估DiI荧光注射开卷准备在卵的电
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations
Please note that all translations are automatically generated. Click here for the English version.

基于miRNA的质粒在发展中的神经管和表型的评估DiI荧光注射开卷准备在卵的电

DOI:
10.3791/4384-v

•

12:41 min

•

October 16, 2012

•

,
1Institute of Molecular Life Sciences,University of Zurich

Chapters

  • 00:05Title
  • 01:15Preparing Eggs for Electroporation
  • 02:59Electroporation of miRNA
  • 04:49Dissection of Embryos for Spinal Cord Preparations
  • 07:28Fixation of Open-books
  • 08:11DiI Injection into Commissural Neurons
  • 09:48Results: Promoter-driven Cell-type Specific Expression in Neural Tube Cross-section and Open-book Preparations
  • 12:12Conclusion

Summary

Automatic Translation

Automatic Translation

可以下调基因的表达在神经管的方法是，在特定的细胞类型，可跟踪的方式进行描述。我们演示了如何<em在OVO</em>电的小分子RNA的质粒，引起时空控制的RNA干扰可用于研究在发展中的神经管连合轴突导向。

Tags

Ovo ElectroporationMiRNA-based PlasmidsDeveloping Neural TubePhenotypesDiI InjectionOpen-book PreparationsCommissural DI1 NeuronsAxon GuidanceDorsal Spinal CordFloorplateRostral TurnBrain ProjectionAttractive And Repulsive Guidance CuesGene Knockdown In VivoChicken Neural TubeCell Type-specific Promoters/enhancersFluorescent Protein MarkerMiR30-RNAi Transcript

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

睾酮和社会决策，结合行为内分泌和实验经济学：

在发展中国家斑马鱼的前脑的神经祖细胞克隆相关的定时实时成像

小鼠视网膜神经节细胞的转染在体内电穿孔

在发展中国家小鼠视网膜体内电穿孔

转基因小鼠内耳的发展<em>在体内</em>电穿孔

高分辨率实时成像发展中的神经上皮细胞的行为

扩展胚胎及成体神经干细胞<em>在子宫内</em电穿孔或病毒立体定向注射

多单位记录方法来描述蝗虫的神经活动（<em> Schistocerca美洲</em>）嗅觉电路

遗传操纵鼠标的发展下丘脑通过<em>在子宫内</em>电穿孔

完整的犁鼻器和副嗅球的体外制剂

Read Article