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基于miRNA的质粒在发展中的神经管和表型的评估DiI荧光注射开卷准备在卵的电
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations
DOI:

12:41 min

October 16, 2012

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Chapters

  • 00:05Title
  • 01:15Preparing Eggs for Electroporation
  • 02:59Electroporation of miRNA
  • 04:49Dissection of Embryos for Spinal Cord Preparations
  • 07:28Fixation of Open-books
  • 08:11DiI Injection into Commissural Neurons
  • 09:48Results: Promoter-driven Cell-type Specific Expression in Neural Tube Cross-section and Open-book Preparations
  • 12:12Conclusion

Summary

Automatic Translation

可以下调基因的表达在神经管的方法是,在特定的细胞类型,可跟踪的方式进行描述。我们演示了如何<em在OVO</em>电的小分子RNA的质粒,引起时空控制的RNA干扰可用于研究在发展中的神经管连合轴突导向。

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