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局部应用药物研究烟碱型乙酰胆碱受体功能的小鼠脑片
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
DOI:

10:04 min

October 29, 2012

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Chapters

  • 00:05Title
  • 01:46Acute Brain Slice Preparation and Patch Clamp Recording
  • 03:47Local Application of Drugs to Neurons in Slices
  • 06:07Controlling the Drug-filled Micropipette with a Piezoelectric Translator
  • 07:22Results: Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
  • 09:33Conclusion

Summary

Automatic Translation

在本文中,我们描述了一个有用的方法来研究神经元的急性分离的大脑切片配体门控离子通道的功能。这种方法涉及使用的药物填充微量移液器为本地应用程序使用标准的膜片钳技术记录神经元的药物。

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