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10:39 min
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June 12, 2013
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我々は内膜破裂を追跡するための方法を説明することは細胞内細菌によって誘発<em>赤痢菌<em></em</em>と<em<em><em>結核菌</em></em</em宿主細胞侵入時>。我々のアッセイはCCF4を利用し、細胞質のホストは、ライブまたは固定細胞におけるFRETプローブ。この記者は、細菌表面上の酵素活性が存在することによって分解される。
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Cite this Article
Keller, C., Mellouk, N., Danckaert, A., Simeone, R., Brosch, R., Enninga, J., Bobard, A. Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens. J. Vis. Exp. (76), e50116, doi:10.3791/50116 (2013).
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