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Naive voksne stamceller Isolering fra primære humane fibroblastkulturer
JoVE Journal
Biology
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JoVE Journal Biology
Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures
DOI:

07:39 min

May 03, 2013

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Chapters

  • 00:05Title
  • 01:27SKP Isolation from Primary Fibroblast Cultures
  • 02:11Sphere Culture Conditions
  • 02:57Immunocytochemistry for Stem Cell Markers
  • 03:59Realtime PCR Analysis of Expression Levels of Stem Cell Markers
  • 05:01Directed Differentiation into Smooth Muscle Cells
  • 05:54Results: SKP Spheres from Primary Fibroblast Cultures
  • 07:16Conclusion

Summary

Automatic Translation

Vi rapporterer en metode til at isolere naive multipotente hud-afledt precursor (SKP) celler fra primære humane fibroblastkulturer. Vi viser, at disse SKPs stammer fra fibroblastkulturer deler lignende stamceller egenskaber til dem stammer direkte fra humane hudbiopsier. Disse celler udtrykker neuralkam markering, nestin, ud over de multipotente markører, såsom OCT4 og Nanog.

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