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Neonatal subventricular Zone Electroporation
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Neonatal Subventricular Zone Electroporation
DOI:

08:06 min

February 11, 2013

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Chapters

  • 00:05Title
  • 01:39Preparation of DNA, Solutions, and Glass Pipettes
  • 02:54Animal Preparation, Glass Pipette Loading and Plasmid Injection
  • 05:04Electroporation and Recovery
  • 06:44Results: Images of an Electroporated Subventricular Zone
  • 07:48Conclusion

Summary

Automatic Translation

Vi viser en minimalt invasiv teknikk kalt neonatal subventricular sone electroporation. Teknikken består i innsprøyting plasmid DNA inn i laterale ventrikkel av neonatale valper og tilføre elektrisk strøm for å levere og genetisk manipulere nevrale stamceller

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