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分离,培养和成年小鼠Cardiomyoctyes的功能分析
JoVE Journal
Biology
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JoVE Journal Biology
Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes
DOI:

12:49 min

September 24, 2013

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Chapters

  • 00:05Title
  • 01:33Cardiomyocyte Isolation
  • 07:32Operating the MMSYS System
  • 10:43Cardiomyocyte Morphology and MMSYS System Analysis
  • 12:28Conclusion

Summary

Automatic Translation

在这里,我们描述了使用Langendorff灌流系统成年小鼠cardiomyoctyes隔离。所得到的细胞是钙耐受性,电静态和可培养和转染腺病毒或慢病毒操纵基因的表达。它们的功能也可以使用MMSYS系统和膜片钳技术进行分析。

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