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利用双水相系统的细胞共培养图案
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Cell Co-culture Patterning Using Aqueous Two-phase Systems
Please note that all translations are automatically generated.
Click here for the English version.
利用双水相系统的细胞共培养图案
DOI:
10.3791/50304-v
•
10:11 min
•
March 26, 2013
•
John P. Frampton
,
Joshua B. White
,
Abin T. Abraham
,
Shuichi Takayama
2
1
Department of Biomedical Engineering
,
University of Michigan
,
2
Department of Macromolecular Science and Engineering
,
University of Michigan
Chapters
00:05
Title
01:32
Phase System Characterization: Determining Thresholds for Phase Separation
03:22
Configuration 1: Exclusion Patterning of HeLa Cells
05:24
Configuration 2: Island Patterning
06:41
Configuration 3: Exclusion / Island Patterned Co-Cultures
08:08
Results: Cell Patterning with the Aqueous Two-phase System
09:47
Conclusion
Summary
Automatic Translation
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Automatic Translation
双水相系统,用于同时图案多个的细胞群体。此快速和容易的方法,细胞图案利用葡聚糖和聚乙二醇的水溶液,和两者之间的聚合物溶液中存在的界面张力的相分离。
Tags
Cell Patterning
Aqueous Two-phase Systems
Dextran
Polyethylene Glycol
Cell Co-culture
Cell Migration Assays
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