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Neuroscience
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立体定向注射病毒载体的基因操作在小鼠脊髓条件
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord
Please note that all translations are automatically generated.
Click here for the English version.
立体定向注射病毒载体的基因操作在小鼠脊髓条件
DOI:
10.3791/50313-v
•
08:39 min
•
March 18, 2013
•
Perrine Inquimbert
,
Martin Moll
,
Tatsuro Kohno
,
Joachim Scholz
1
Département Nociception et Douleur, Institut des Neurosciences Cellulaires et Intégratives
,
Centre National de la Recherche Scientifique (CNRS)
,
2
Departments of Anesthesiology and Pharmacology
,
Columbia University
,
3
Department of Anesthesiology
,
Niigata University Graduate School of Medical and Dental Sciences
Chapters
00:05
Title
01:27
Preparation of Equipment and Virus Particle Suspension
03:09
Laminectomy
04:57
Injection
06:21
Wound Closure and Postoperative Care
07:35
Results: Transfection of Dorsal Horn Neurons in the Mouse Spinal Cord
08:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
病毒载体可以进行有针对性的基因操作。我们提出了一个条件性基因的表达或消融方法在小鼠脊髓,采用立体的病毒载体注射到脊髓背角,初级躯体感觉传入中枢神经系统的神经元之间的突触接触的一个突出的部位。
Tags
Gene Manipulation
Stereotaxic Injection
Viral Vector
Mouse Spinal Cord
Conditional Gene Expression
Gene Silencing
Dorsal Horn
Laminectomy
Adeno-associated Virus
Reporter Gene
EGFP
Transfection
Article
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