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Stereotaktisk injektion af en virusvektor til Betinget Gene Manipulation i Mouse Spinal Cord
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1Département Nociception et Douleur, Institut des Neurosciences Cellulaires et Intégratives,Centre National de la Recherche Scientifique (CNRS), 2Departments of Anesthesiology and Pharmacology,Columbia University , 3Department of Anesthesiology,Niigata University Graduate School of Medical and Dental Sciences
Chapters
- 00:05Title
- 01:27Preparation of Equipment and Virus Particle Suspension
- 03:09Laminectomy
- 04:57Injection
- 06:21Wound Closure and Postoperative Care
- 07:35Results: Transfection of Dorsal Horn Neurons in the Mouse Spinal Cord
- 08:08Conclusion
Virusvektorer på målrettet genmanipulation. Vi viser en fremgangsmåde til betinget genekspression eller ablation i muse rygmarv under anvendelse af stereotaktisk injektion af en viral vektor i den dorsale horn, en fremtrædende sted for synaptisk kontakt mellem primære somatosensoriske afferenter og neuroner i centralnervesystemet.
