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En<em> In vitro</em> Model til Studieinformationen Heterogeneity for Human Makrofag Differentiering og polarisering
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Immunology and Infection
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JoVE Journal Immunology and Infection
An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

En<em> In vitro</em> Model til Studieinformationen Heterogeneity for Human Makrofag Differentiering og polarisering

DOI:
10.3791/50332-v

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07:42 min

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June 12, 2013

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, , , , , , ,
1Department of Cardiology,University of Heidelberg

Chapters

  • 00:05Title
  • 01:09PBMC Isolation (Histopaque) and Cell Counting
  • 02:55Negative Isolation of Monocytes
  • 05:09Results: Representative Primary Human Macrophages
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

Monocytafledte makrofager er vigtige celler i det medfødte immunsystem. Her beskriver vi en nem at bruge<em> In vitro</em> Model til at generere disse celler. Brug gradientcentrifugering, negativ perle isolation og særlige celledyrkningsbetingelser, kan monocytafledte makrofager Der genereres fænotypiske og funktionelle studier.

Tags

Macrophage DifferentiationMacrophage PolarizationIn Vitro ModelPeripheral Blood Mononuclear CellsMonocyte IsolationMacrophage CultureHuman MacrophagesSpecies-specific Differences

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