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Isolamento de fluido cerebrospinal de embriões de roedores para uso com dissecados Cerebral Cortical Explantes
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1Department of Physical Medicine and Rehabilitation,VA Greater Los Angeles Healthcare System, 2Department of Pharmacology and Physiology, Institute for Neuroscience,The George Washington University School of Medicine and Health Sciences, 3Division of Genetics, Department of Medicine,Boston Children’s Hospital, 4Howard Hughes Medical Institute,Boston Children’s Hospital, 5Department of Pathology,Boston Children’s Hospital, Harvard Medical School
Chapters
- 00:05Title
- 01:24Micro-capillary Pipette and Embryo Preparations
- 02:47Ventricular CSF Collection
- 05:01Cortical Explant Dissection
- 06:51Transferring and Culturing of Cortical Explant in CSF
- 08:30CSF Collection Characteristics
- 09:20Conclusion
O ventricular líquido cefalorraquidiano (LCR) banha os neuroepiteliais e cerebral células progenitoras corticais durante o desenvolvimento do cérebro no início do embrião. Aqui descrevemos o método desenvolvido para isolar CSF ventricular de embriões de roedores de idades diferentes, a fim de investigar a sua função biológica. Além disso, podemos demonstrar o nosso dissecção explante cerebral cortical e técnica de cultura que permite o crescimento de explantes com volumes mínimos de meio de cultura ou do LCR.
