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获取荧光时间推移电影的芽殖酵母和分析单细胞动力学移植物
JoVE Journal
Biology
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JoVE Journal Biology
Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS
DOI:

17:01 min

July 18, 2013

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Chapters

  • 00:05Title
  • 01:36Obtain Fluorescent Microscopy Movies of Single Yeast Cells Growing in a Microfluidic Chamber
  • 06:33Format and Segment Data for Tracking Using the FormatData GUI in MATLAB
  • 09:00Track Cells and Lineages through Time with ProcessTimeSeries GUI, and Curate ID and Lineage Assignments
  • 12:33Exporting Data for Analysis
  • 13:35Results: Analysis of Single-cell Time Series
  • 16:22Conclusion

Summary

Automatic Translation

我们提出了一个简单的协议来获得生长的酵母细胞的荧光显微镜的电影,和一个基于GUI的软件程序包,提取单细胞的时间序列数据。该分析包括自动沿袭和集成的跟踪数据的目视检查和手工curation分组表决的时间分配。

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