A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Institute for Complex Molecular Systems & Laboratory of Macromolecular and Organic Chemistry,Eindhoven University of Technology & NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 2NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR & IIT@NEST,Center for Nanotechnology Innovation, 3NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 4IIT@NEST,Center for Nanotechnology Innovation
Chapters
- 00:05Title
- 02:30Synthesis of the Sensors
- 04:20In vitro pH Measurements
- 05:36Cell Culture and Electroporation
- 06:38pH Sensing in Living HeLa Cells
- 08:47In vivo Sample Preparation
- 10:30pH Imaging in Mouse Brain
- 12:24Representative Analysis of Dendrimer-based pH Sensor Fluorescence
- 15:45Conclusion
Fluorescentie sensoren zijn krachtige hulpmiddelen in life science. Hier beschrijven we een methode te synthetiseren en te gebruiken dendrimeer gebaseerde fluorescente sensoren pH meten in levende cellen en in vivo. De dendritische steiger versterkt de eigenschappen van geconjugeerde fluorescente kleurstoffen leidt tot betere detectie eigenschappen.
