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1Institute for Complex Molecular Systems & Laboratory of Macromolecular and Organic Chemistry,Eindhoven University of Technology & NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 2NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR & IIT@NEST,Center for Nanotechnology Innovation, 3NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 4IIT@NEST,Center for Nanotechnology Innovation
Chapters
- 00:05Title
- 02:30Synthesis of the Sensors
- 04:20In vitro pH Measurements
- 05:36Cell Culture and Electroporation
- 06:38pH Sensing in Living HeLa Cells
- 08:47In vivo Sample Preparation
- 10:30pH Imaging in Mouse Brain
- 12:24Representative Analysis of Dendrimer-based pH Sensor Fluorescence
- 15:45Conclusion
Fluorescens sensorer er kraftige verktøy i life science. Her beskriver vi en metode for å syntetisere og bruke dendrimer-baserte fluorescerende sensorer for å måle pH-verdien i levende celler, og in vivo. Dendrittiske stillas forbedrer egenskapene til konjugerte fluorescerende fargestoffer som fører til bedre føle egenskaper.
