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Bioengineering
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一种多功能自动化平台微尺度细胞刺激实验
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
Please note that all translations are automatically generated.
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一种多功能自动化平台微尺度细胞刺激实验
DOI:
10.3791/50597-v
•
12:21 min
•
August 06, 2013
•
Anupama Sinha
,
Mais J. Jebrail
,
Hanyoup Kim
3
,
Kamlesh D. Patel
,
Steven S. Branda
1
Department of Systems Biology
,
Sandia National Laboratories
,
2
Department of Biotechnology and Bioengineering
,
Sandia National Laboratories
,
3
Canon U.S. Life Sciences
,
4
Department of Advanced Systems Engineering and Deployment
,
Sandia National Laboratories
Chapters
00:05
Title
01:59
Assembly and Population of the Cell Perfusion Chambers
04:28
Fabrication of the Digital Microfluidic (DMF) Module and Assembly of the Hub Architecture
06:37
Stimulation of Cell Populations
07:45
Terminate Stimulation and Recovery of Cell Lysates
09:19
Results: Micro-scale Cell Stimulation Produces Results Similar to Conventional Cultures
11:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
微观尺度细胞刺激实验,我们已经开发了一个自动细胞培养和审讯平台。该平台提供简单,功能多样,培育和刺激小的细胞群体,并恢复裂解分子分析和精确的控制。该平台非常适合使用珍贵的细胞和/或试剂的研究。
Tags
Automated Platform
Micro-scale Cell Stimulation
In Vitro Analysis
Micro-scale Cultures
Digital Microfluidics
Cell Perfusion Chambers
Cell Culture
Immune Cell Response
Transcriptional Responses
Reagent Consumption
Experiment Variability
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