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Um chip microfluídico para a Versátil Análise Química de células individuais
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
DOI:

15:41 min

October 15, 2013

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Chapters

  • 00:05Title
  • 02:13Fabrication of the Microfluidic Chip
  • 05:31Chip Preparation for Enzymatic Assays
  • 07:15Additional Chip Preparation for Immunoassays
  • 10:05Cell Harvesting and Glucose 6-Phosphate Dehydrogenase Assay
  • 12:08Results: Enzymatic and Antibody Analysis of Single Cells
  • 15:08Conclusion

Summary

Automatic Translation

Neste artigo apresentamos um chip microfluídico para análise única célula. Ele permite a quantificação de proteínas intracelulares, enzimas, co-factores, e os segundos mensageiros, por meio de ensaios fluorescentes ou imunoensaios.

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