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Motor Nerve transection og Time-lapse Imaging af gliacelle Behaviors Live zebrafisk
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Motor Nerve Transection and Time-lapse Imaging of Glial Cell Behaviors in Live Zebrafish
DOI:

09:05 min

June 20, 2013

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Chapters

  • 00:05Title
  • 01:18Preparation and Mounting of Zebrafish Embryos
  • 03:10Laser Calibration and Testing
  • 04:35Nerve Transection Using Laser Ablation and Time-lapse Confocal Imaging of Glial Cell Behaviors
  • 06:39Representative Laser Ablation Results
  • 08:39Conclusion

Summary

Automatic Translation

Selvom det perifere nervesystem (PNS), er i stand til væsentlig reparation efter skade, er lidt om de cellulære og molekylære mekanismer, der styrer dette fænomen. Ved hjælp af levende, transgene zebrafisk og en reproducerbar nerve transection assay, kan vi studere dynamiske glialcellelinjer adfærd under nerve degeneration og regeneration.

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