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JoVE Journal
Chemistry

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基质辅助飞行的完整蛋白质(MALDI-TOF)质谱分析激光解吸/电离飞行时间大于100 kDa的
 

基质辅助飞行的完整蛋白质(MALDI-TOF)质谱分析激光解吸/电离飞行时间大于100 kDa的

Article doi: 10.3791/50635
September 9th, 2013

Summary September 9th, 2013

Please note that all translations are automatically generated.

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精确质量测定代表蛋白质的调查过程中的重要一步。基质辅助激光解吸/电离(MALDI)质谱(MS)可用于这种确定和其主要优点是耐受的盐,去垢剂和污染物。在这里,我们示出了用于蛋白质大于100 kDa的通过MALDI-MS较大的分析可访问的方法。

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