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Chapters
- 00:05Title
- 00:54His-trap Purification
- 02:24Nuclease Assay
- 04:14Results: The Exonuclease Activity of DmWRNexo
- 05:51Conclusion
Exonucleases play critical roles in ensuring genome stability. Loss of WRN exonuclease function results in premature aging. Studying substrates and other requirements of the nuclease in vitro can help elucidate its role in vivo. Here we demonstrate a rapid and reproducible fluorescence-based assay to measure its nuclease activity.
