Journal
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Lo studio delle interazioni di<em> Staphylococcus aureus</em> Con neutrofili mediante citometria a flusso e Time Lapse Microscopy
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy
DOI:

10:40 min

July 17, 2013

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Chapters

  • 00:05Title
  • 01:32Density Gradient Separation of Polymorphonuclear Neutrophils (PMNs) from Human Blood
  • 04:31Flow Cytometric for Assessment of Calcium Mobilization in Human PMNs
  • 06:40Fluorescence Microscopy Analysis of Bacteria GFP Expression after Phagocytosis by PMNs
  • 08:51Results: Representative Analyses of PSMα Activation in Human PMNs
  • 10:06Conclusion

Summary

Automatic Translation

Presenteremo i metodi per studiare l'effetto di PSMs e altre tossine secrete da<em> Staphylococcus aureus</em> Sui neutrofili mediante citometria a flusso e microscopia a fluorescenza.

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