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使用氢交换质谱分析蛋白质动力学
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
DOI:

11:37 min

November 29, 2013

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Chapters

  • 00:05Title
  • 02:39Preparation for Amide Hydrogen-exchange and Hydrogen Exchange Mass Spectrometry (HX-MS)
  • 04:09Determination of Peptic Peptides Using MS/MS Spectra
  • 05:54Identification of Protein-protein Interaction Interfaces
  • 08:48Results: Analysis of Protein Dynamics
  • 11:04Conlusion

Summary

Automatic Translation

蛋白质构象和动力学的关键是理解蛋白质结构与功能之间的关系。氢交换耦合用高分辨率质谱法是研究蛋白质的构象动力学以及表征蛋白质 - 配体和蛋白质之间的相互作用,其中包括接触接口和变构效应的灵活的方法。

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