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使用氢交换质谱分析蛋白质动力学
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JoVE Journal
Chemistry
Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
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使用氢交换质谱分析蛋白质动力学
DOI:
10.3791/50839-v
•
11:37 min
•
November 29, 2013
•
Nikolai Hentze
,
Matthias P. Mayer
1
Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH)
,
University of Heidelberg
Chapters
00:05
Title
02:39
Preparation for Amide Hydrogen-exchange and Hydrogen Exchange Mass Spectrometry (HX-MS)
04:09
Determination of Peptic Peptides Using MS/MS Spectra
05:54
Identification of Protein-protein Interaction Interfaces
08:48
Results: Analysis of Protein Dynamics
11:04
Conlusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
蛋白质构象和动力学的关键是理解蛋白质结构与功能之间的关系。氢交换耦合用高分辨率质谱法是研究蛋白质的构象动力学以及表征蛋白质 - 配体和蛋白质之间的相互作用,其中包括接触接口和变构效应的灵活的方法。
Tags
Hydrogen Exchange
Mass Spectrometry
Protein Dynamics
Protein Conformation
Protein Folding
Protein-protein Interactions
Enzyme Catalysis
Allostery
Structural Analysis
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