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海胆受精卵的高通量显微注射
JoVE Journal
Biology
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JoVE Journal
Biology
High Throughput Microinjections of Sea Urchin Zygotes
Please note that all translations are automatically generated.
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海胆受精卵的高通量显微注射
DOI:
10.3791/50841-v
•
12:40 min
•
January 21, 2014
•
Nadezda A. Stepicheva
,
Jia L. Song
1
Department of Biological Sciences
,
University of Delaware
Chapters
00:05
Title
01:26
Experimental Preparations
04:00
Collecting Sea Urchin Gametes
06:12
Rowing the Eggs
07:40
Microinjection of the Sea Urchin Zygotes
11:13
Injection of Reporter Constructs Does Not Affect Development
12:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
显微注射是用于递送DNA构建体,mRNA表达,吗啉代反义寡核苷酸或其它处理成卵,胚胎,以及不同物种的细胞中的常见技术。
Tags
Microinjection
Sea Urchin Zygotes
High-throughput
DNA Constructs
MRNAs
Recombinant Proteins
Gain Of Function
Loss Of Function
Fluorescent Dye
Colorimetric Dye
Cell Delivery
Embryo Delivery
Biological Processes
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