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Biology

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JoVE Journal Biology
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FtsZ Polymerization Assays: Simple Protocols and Considerations

Article DOI: 10.3791/50844
November 16th, 2013 •
1, 1
1Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen

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November 16th, 2013

Polymerization of FtsZ is essential for bacterial cell division. In this report, we detail simple protocols to monitor FtsZ polymerization activity and discuss the influence of buffer composition. The protocols can be used to study the interaction of FtsZ with regulatory proteins or antibacterial drugs that affect FtsZ polymerization.

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