FtsZ Polymerization Assays: Simple Protocols and Considerations

Journal

Biology

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

FtsZ Polymerization Assays: Simple Protocols and Considerations

DOI:

10.3791/50844-v

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12:04 min

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November 16, 2013

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Ewa Król, Dirk-Jan Scheffers

¹Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute,University of Groningen

Chapters

00:05Title
01:20FtsZ Sedimentation Assay at Slow Spin
03:2990° Light Scattering
05:19GTP Hydrolysis Assay
09:33Results: Buffer Influence on Tubule Formation
11:40Conclusion

Summary

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Polymerization of FtsZ is essential for bacterial cell division. In this report, we detail simple protocols to monitor FtsZ polymerization activity and discuss the influence of buffer composition. The protocols can be used to study the interaction of FtsZ with regulatory proteins or antibacterial drugs that affect FtsZ polymerization.

FtsZ Polymerization Assays: Simple Protocols and Considerations

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