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Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus
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Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus
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Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus

DOI:
10.3791/50848-v

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08:09 min

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December 19, 2013

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, , , ,
1Physiology Course,Marine Biological Laboratory, 2Department of Biochemistry & Biophysics,University of California San Francisco, 3Department of Physics and Astronomy,Vrije Universiteit Amsterdam, 4Max Planck Institute of Molecular Cell Biology and Genetics, 5Department of Physics,University of Illinois Urbana-Champaign

Chapters

  • 00:05Title
  • 01:12Cell Culture and Collection
  • 02:32Preparation of Tools and Cells for Microsurgery
  • 03:43High Resolution Imaging of Wound Healing in Live Stentor Cells
  • 05:19Analyzing Cytoplasmic Flow During Wound Healing
  • 07:13Results: Live Imaging of Stentor Cells Following Disruption
  • 07:33Conclusion

Summary

Automatic Translation

Automatic Translation

The giant ciliate Stentor coeruleus is a classical system for studying regeneration and wound healing in single cells. By imaging Stentor cells simultaneously at low and high magnification it is possible to measure cytoplasmic flows before, during, and after wounding.

Tags

Keywords: Stentor CoeruleusSingle-cell Wound HealingCytoplasmic FlowDouble-decker MicroscopeHigh-resolution ImagingCell Membrane RepairClassical Model Organism

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