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Induktion av invasiva Transitional Cell blåskarcinom i immuna intakt human MUC1 transgena möss: En modell för immunterapi utveckling
JoVE Journal
Medicine
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JoVE Journal Medicine
Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
DOI:

11:02 min

October 30, 2013

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Chapters

  • 00:05Title
  • 01:43Bladder Cancer Induction and Vaccination Treatments
  • 02:49Monitoring and Sample Collection
  • 04:11Multiplex Fluorometric Microbead Immunoassay
  • 06:37IFN-γ/IL-4 ELISpot Preparation and Analysis
  • 08:07Representative Transitional Cell Bladder Carcinoma Induction Results
  • 10:39Conclusion

Summary

Automatic Translation

En N-butyl-N-(4-hydroxibutyl) nitrosamin-inducerad blåscancer modell utvecklades i human mucin 1 (MUC1) transgena möss för att testa MUC1-riktad immunterapi. Efter administrering av en MUC1-riktad peptidvaccin, var en cytotoxisk T lymfocytsvaret MUC1 bekräftades genom mätning av serum cytokinnivåer och T-cell specifik aktivitet.

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