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MexAB外排泵从铜绿假单胞菌体外研究
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa
DOI:

13:40 min

February 17, 2014

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Chapters

  • 00:05Title
  • 03:53Protein Production and Purification
  • 05:24Preparation of Proteoliposomes
  • 08:35Assessment of the Reconstitution Efficacy on Sucrose Gradient
  • 09:37Fluorescence Staining
  • 10:28Monitoring Pyranine Fluorescence as a Function of Time While a Proton Gradient is Generated
  • 12:40Conclusion

Summary

Automatic Translation

我们提出了一个协议,用于在体外研究铜绿假单胞菌外排泵。该协议允许对脂质体膜中的健壮的,可逆的,可调谐的质子梯度的产生,因此,应能适应由protomotive力激励任何膜蛋白。

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