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在 MexAB外排泵从铜绿假单胞菌的体外研究

JoVE Journal
Biology

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JoVE Journal Biology

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

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在 MexAB外排泵从铜绿假单胞菌的体外研究

DOI:

10.3791/50894-v

•

13:40 min

•

February 17, 2014

•

Alice Verchère, Manuela Dezi, Isabelle Broutin, Martin Picard

¹Laboratoire de Cristallographie & RMN Biologiques,CNRS & Université Paris Descartes

Chapters

00:05Title
03:53Protein Production and Purification
05:24Preparation of Proteoliposomes
08:35Assessment of the Reconstitution Efficacy on Sucrose Gradient
09:37Fluorescence Staining
10:28Monitoring Pyranine Fluorescence as a Function of Time While a Proton Gradient is Generated
12:40Conclusion

Summary

Automatic Translation

我们提出了一个协议，用于在体外研究铜绿假单胞菌外排泵。该协议允许对脂质体膜中的健壮的，可逆的，可调谐的质子梯度的产生，因此，应能适应由protomotive力激励任何膜蛋白。

Tags

Membrane Protein Efflux Pump Pseudomonas Aeruginosa MexAB In Vitro Reconstitution Liposome Bacteriorhodopsin Proton Gradient Pyranin Transport Activity RND Transporter

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