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Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy
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Chapters
- 00:05Title
- 01:51Aminosilanization of Glass Cover Slips and Cantilevers
- 04:21Protein Disulfide Reduction
- 05:36PEGylation of Cover Slips and Cantilevers
- 06:53Protein Conjugation and AFM Setup
- 09:21Results: Investigation of a Type I Cohesin-dockerin Pair from C. thermocellum
- 10:37Conclusion
Cellulosomes are multienzyme complexes designed for digesting cellulose. AFM-based SMFS was used to study the mechanical properties and folding configuration of cellulosome-associated protein assemblies. We present a complete workflow for protein immobilization, data acquisition, and data analysis to study the interactions of individual receptor-ligand complexes involved in cellulosome assembly.
