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Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
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Bioengineering
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JoVE Journal Bioengineering
Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

DOI:
10.3791/50980-v

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08:02 min

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November 07, 2013

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, , , , , , , ,
1Department of Chemical Engineering,Massachusetts Institute of Technology, 2David H. Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology

Chapters

  • 00:05Title
  • 01:20Experiment Preparation
  • 02:19Assembly of the Intracellular Delivery Platform
  • 03:28Cell Preparation and Device Operation
  • 06:13Results: Delivery of Dextran into HeLa Cells
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

Rapid mechanical deformation of cells has emerged as a promising, vector-free method for intracellular delivery of macromolecules and nanomaterials. This protocol provides detailed steps on how to use the system for a broad range of applications.

Tags

Cell SqueezingIntracellular DeliveryMicrofluidic DeviceVector-free DeliveryCell Membrane DisruptionPrimary Immune CellsCell ReprogrammingCarbon NanotubesQuantum DotsOptimizationDevice AssemblyCell PreparationSystem Operation

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