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Bioengineering
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电池挤压作为一种强大的,微流控细胞内传送平台
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
Please note that all translations are automatically generated.
Click here for the English version.
电池挤压作为一种强大的,微流控细胞内传送平台
DOI:
10.3791/50980-v
•
08:02 min
•
November 07, 2013
•
Armon Sharei
,
Nahyun Cho
,
Shirley Mao
,
Emily Jackson
,
Roberta Poceviciute
,
Andrea Adamo
,
Janet Zoldan
,
Robert Langer
2
,
Klavs F Jensen
1
Department of Chemical Engineering
,
Massachusetts Institute of Technology
,
2
David H. Koch Institute for Integrative Cancer Research
,
Massachusetts Institute of Technology
Chapters
00:05
Title
01:20
Experiment Preparation
02:19
Assembly of the Intracellular Delivery Platform
03:28
Cell Preparation and Device Operation
06:13
Results: Delivery of Dextran into HeLa Cells
07:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
细胞的快速机械变形已成为细胞内输送大分子和纳米材料的一种很有前途的,矢量-free方法。这个协议提供了关于如何使用该系统进行范围广泛的应用中的详细步骤。
Tags
Cell Squeezing
Intracellular Delivery
Microfluidic Device
Vector-free Delivery
Cell Membrane Disruption
Primary Immune Cells
Cell Reprogramming
Carbon Nanotubes
Quantum Dots
Optimization
Device Assembly
Cell Preparation
System Operation
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