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En forbedret metode til forberedelse af type I kollagen fra hud
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
An Improved Method for the Preparation of Type I Collagen From Skin
DOI:

05:17 min

January 21, 2014

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Chapters

  • 00:05Title
  • 01:10Preparing the Dermal Sample
  • 01:44Removing Noncollagenous Solubilized Material
  • 02:48Type I Collagen Extraction
  • 04:22Results: Rapid Isolation from Dermis to Yield COL1 Polymerized into Fibrils
  • 04:58Conclusion

Summary

Automatic Translation

Traditionelle metoder til isolering af opløselige type 1 kollagen (Kol1) kræver omkring 10 dage fra start til slut på grund af langvarige buffer inkubationer og møjsommelig resuspensions af fibriller. Her beskriver vi et middel til at rense Kol1 fra små dermale biopsier i mindre end 3 timer.

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