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Neuroscience
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小脑颗粒神经元的遗传操作<em>体外</em>和<em>在体内</em>为研究神经元形态和迁移
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Genetic Manipulation of Cerebellar Granule Neurons
In Vitro
and
In Vivo
to Study Neuronal Morphology and Migration
Please note that all translations are automatically generated.
Click here for the English version.
小脑颗粒神经元的遗传操作<em>体外</em>和<em>在体内</em>为研究神经元形态和迁移
DOI:
10.3791/51070-v
•
09:07 min
•
March 17, 2014
•
Anna Holubowska
2
,
Chaitali Mukherjee
2
,
Mayur Vadhvani
2
,
Judith Stegmüller
2
1
Cellular and Molelcular Neurobiology
,
Max Planck Institute of Experimental Medicine
,
2
Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)
Chapters
00:05
Title
01:26
Transfection of Cultured Neurons
04:20
In Vivo
Electroporation
07:10
Results: Genetic Manipulation of Cerebellar Granule Neurons to Study Neuronal Morphology and Migration
08:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
神经元的形态发生和迁移的基本正常的大脑发育至关重要的事件。在这里,我们描述的方法来操纵基因培养的小脑颗粒神经元和小脑发展的形态和神经元的迁徙特性的评估。
Tags
Cerebellar Granule Neurons
Genetic Manipulation
Neuronal Morphogenesis
Neuronal Migration
In Vitro Analysis
In Vivo Analysis
Axonogenesis
Dendritogenesis
RNA Interference
Overexpression
Small Molecules
Rodent Cerebellar Cortex
In Vivo Electroporation
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