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绿色荧光蛋白标记的质膜蛋白质的扩散系数的简易测量采用K空间图像相关光谱
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JoVE Journal
Biology
Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy
Please note that all translations are automatically generated.
Click here for the English version.
绿色荧光蛋白标记的质膜蛋白质的扩散系数的简易测量采用K空间图像相关光谱
DOI:
10.3791/51074-v
•
11:43 min
•
May 10, 2014
•
Eva C. Arnspang
,
Jennifer S. Koffman
,
Saw Marlar
,
Paul W. Wiseman
,
Lene N. Nejsum
1
Institute of Molecular Biology and Genetics and Interdisciplinary Nanoscience Center
,
Aarhus University
,
2
Departments of Chemistry and Physics
,
McGill University
Chapters
00:05
Title
01:15
Import the Image Sequence of Interest into an Imaging Analysis Program
02:37
Perform the Analysis
06:52
Calculate Average of the Diffusion Coefficient
07:56
Results: Example ROI Crops, Diffusion, and k
2
Plots
11:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提供了一个一步一步引导到波动分析技术K空间图像相关光谱(KICS),用于测量扩散系数的荧光在活的哺乳动物细胞标记质膜蛋白。
Tags
Diffusion Coefficients
EGFP
Plasma Membrane Proteins
K-Space Image Correlation Spectroscopy (kICS)
Fluorescence Microscopy
Image Analysis
Aquaporin-3
Membrane Protein Diffusion
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