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隔离与人类心室肌细胞的功能研究从新鲜手术样品
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Medicine
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Medicine
Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples
Please note that all translations are automatically generated.
Click here for the English version.
隔离与人类心室肌细胞的功能研究从新鲜手术样品
DOI:
10.3791/51116-v
•
14:39 min
•
April 21, 2014
•
Raffaele Coppini
,
Cecila Ferrantini
,
Alessandro Aiazzi
,
Luca Mazzoni
,
Laura Sartiani
,
Alessandro Mugelli
,
Corrado Poggesi
,
Elisabetta Cerbai
1
Department NeuroFarBa, Division of Pharmacology
,
University of Florence
,
2
Department of Clinical and Experimental Medicine, Division of Physiology
,
University of Florence
Chapters
00:05
Title
02:47
Collection and Processing of Myocardial Samples
03:52
Washing and Digestion of Myocardial Chunks
06:43
Cell Resuspension and Ca
2+
Readapation
08:28
Functional Evaluation of Isolated Cardiomyocytes
11:11
Results
13:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
心脏疾病的细胞基础上的现有知识大多依赖于对动物模型的研究。在这里,我们描述和验证了一种新的方法从人心肌小型手术样品得到单一可行的心肌细胞。人心肌细胞可用于电生理学研究和药物测试。
Tags
Cardiomyocytes
Ventricular Myocardium
Cardiac Surgery
Electrophysiology
Intracellular Calcium
Cardiac Disease
Cellular And Molecular Basis
Therapeutic Targets
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