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烟草中瞬时表达的蛋白作为一个案例研究:复杂系统采用的实验方法设计的表征
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study
Please note that all translations are automatically generated.
Click here for the English version.
烟草中瞬时表达的蛋白作为一个案例研究:复杂系统采用的实验方法设计的表征
DOI:
10.3791/51216-v
•
20:24 min
•
January 31, 2014
•
Johannes Felix Buyel
,
Rainer Fischer
2
1
Institute for Molecular Biotechnology
,
RWTH Aachen University
,
2
Institute for Molecular Biology and Applied Ecology
,
Fraunhofer Gesellschaft
Chapters
00:05
Title
01:13
Planning a DoE Strategy
03:15
Plant Cultivation
04:12
Transient Protein Expression
07:02
Protein Quantitation
08:35
Data Analysis and Evaluation
11:53
Results: The DoE Strategy Examines Major Factors and Incubation Conditions that Impact Transient Expression of Proteins in Plants
20:01
Conclusion
Summary
Automatic Translation
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我们描述的实验方法设计,可以被用来确定与模型的转基因的调控元件,植物生长和发育参数和培养条件对植物中的单克隆抗体和报告蛋白的瞬时表达的影响。
Tags
Design Of Experiments
Transient Protein Expression
Tobacco Plants
Biopharmaceuticals
Anti-HIV Monoclonal Antibody
DsRed
Complexity Reduction
Software-guided Experiment Design
Predictive Models
Mechanistic Models
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