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烟草中瞬时表达的蛋白作为一个案例研究:复杂系统采用的实验方法设计的表征
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study
DOI:

20:24 min

January 31, 2014

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Chapters

  • 00:05Title
  • 01:13Planning a DoE Strategy
  • 03:15Plant Cultivation
  • 04:12Transient Protein Expression
  • 07:02Protein Quantitation
  • 08:35Data Analysis and Evaluation
  • 11:53Results: The DoE Strategy Examines Major Factors and Incubation Conditions that Impact Transient Expression of Proteins in Plants
  • 20:01Conclusion

Summary

Automatic Translation

我们描述的实验方法设计,可以被用来确定与模型的转基因的调控元件,植物生长和发育参数和培养条件对植物中的单克隆抗体和报告蛋白的瞬时表达的影响。

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