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Rapid Analysis and Exploration of Fluorescence Microscopy Images
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JoVE Journal Biology
Rapid Analysis and Exploration of Fluorescence Microscopy Images

Rapid Analysis and Exploration of Fluorescence Microscopy Images

DOI:
10.3791/51280-v

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11:41 min

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March 19, 2014

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, , , , , ,
1Green Center for Systems Biology,UT Southwestern Medical Center, 2Advanced Imaging Research Center,UT Southwestern Medical Center, 3Princeton University

Chapters

  • 00:05Title
  • 01:36Sample preparation and Imaging
  • 02:23Image Analysis
  • 07:02Exploring Results
  • 09:28Results: Grouping Drugs Based on Their Mechanism of Action
  • 11:10Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a workflow for rapidly analyzing and exploring collections of fluorescence microscopy images using PhenoRipper, a recently developed image-analysis platform.

Tags

Keywords: Fluorescence MicroscopyImage AnalysisCell SegmentationPhenoRipperHigh-throughput MicroscopyImage-based AssaysQuantitative AnalysisPhenotypic ExplorationScreeningAssay OptimizationData Visualization

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