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Journal
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Immunology and Infection
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生物能量和氧化突发:协议用于人类白细胞和血小板的隔离与评价
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets
Please note that all translations are automatically generated.
Click here for the English version.
生物能量和氧化突发:协议用于人类白细胞和血小板的隔离与评价
DOI:
10.3791/51301-v
•
11:40 min
•
March 27, 2014
•
Philip A. Kramer*
1
,
Balu K. Chacko*
1
,
Saranya Ravi
,
Michelle S. Johnson
,
Tanecia Mitchell
,
Victor M. Darley-Usmar*
1
1
UAB Mitochondrial Medicine Laboratory, Center for Free Radical Biology, Department of Pathology
,
University of Alabama at Birmingham
Chapters
00:05
Title
02:33
Cell Isolation and Plating
08:02
Results: Representative Bioenergetic Index Analyses
10:11
Conclusion
Summary
Automatic Translation
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Automatic Translation
血液白细胞和血小板可以作为一个单独的整体生物能量健康的一个标志,所以具有监视病理过程和治疗的影响的可能性。这里我们描述的方法来分离并测量线粒体功能和在这些细胞中的氧化爆发。
Tags
Bioenergetics
Oxidative Burst
Leukocytes
Platelets
Mitochondrial Dysfunction
Atherosclerosis
Diabetes
Septic Shock
Neurodegenerative Diseases
Hyperglycemia
Inflammation
Magnetic Bead Isolation
Cell Adhesion
Microplate Respirometry
Extracellular Flux Analyzer
Monocytes
Lymphocytes
Neutrophils
Reactive Oxygen Species
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