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Bioengineering
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无细胞的转录和翻译的机械囊泡的封装建设模拟蜂窝
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
The Encapsulation of Cell-free Transcription and Translation Machinery in Vesicles for the Construction of Cellular Mimics
Please note that all translations are automatically generated.
Click here for the English version.
无细胞的转录和翻译的机械囊泡的封装建设模拟蜂窝
DOI:
10.3791/51304-v
•
10:28 min
•
October 21, 2013
•
Amy C. Spencer
,
Paola Torre
,
Sheref S. Mansy
1
Centre for Integrative Biology
,
University of Trento
Chapters
00:05
Title
01:29
Preparing the Thin Lipid Film
02:57
Lipid Resuspension and Vesicle Homogenization
04:00
Vesicle Extrusion and Lyophilization
06:38
Encapsulating Transcription-translation Machinery
08:14
Microscopy
08:52
Results: Fluorescent Microscopy Images
09:54
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们介绍简单的方法编制泡,封装的转录和翻译机制,监测蛋白质的生产。将得到的无细胞系统可以用来作为一个起点,从而建立越来越复杂的细胞模仿。
Tags
Cell-free Transcription
Cell-free Translation
Vesicles
Cellular Mimics
Bottom-up
Compartmentalization
Water-in-oil Emulsions
Microfluidics
Phospholipid Vesicles
Recombinant Protein Expression
Genetic Circuitry
Minimum Requirements For Cellular Life
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