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Tissue Engineering af Tumor Stromal mikromiljø med Application til cancercelleinvasion
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Bioengineering
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JoVE Journal Bioengineering
Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion

Tissue Engineering af Tumor Stromal mikromiljø med Application til cancercelleinvasion

DOI:
10.3791/51321-v

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05:48 min

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March 18, 2014

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,
1Division of Cancer Research, Ninewells Hospital and Medical School,University of Dundee, 2Institute of Medical Biology,A*Star, Singapore

Chapters

  • 00:05Title
  • 01:20In Vitro Construction of Fibroblast-derived Native Matrix
  • 03:01Invasion Assay with Tumor Squamous Cell Carcinoma (SCC) Keratinocytes
  • 04:47Results: Representative cSCC Invasion into the Native Matrix
  • 05:24Conclusion

Summary

Automatic Translation

Automatic Translation

Manipuleret væv fibroblast-afledte native matrix er en ny værktøj til at generere en stromal substrat, som understøtter epitelcelleproliferation og differentiering. Her en protokol at anvende denne metode til at vurdere virkningerne af forskellige stromale celletyper på tumor cellebiologi præsenteres.

Tags

Tumor Stromal Microenvironment3D Organotypic CulturesExtracellular MatrixType I CollagenEngelbreth-Holm-Swarm Mouse SarcomaDermal FibroblastsRecessive Dystrophic Epidermolysis BullosaNative MatrixEpithelial-mesenchymal Cell InteractionsType VII CollagenTumor Cell Invasion

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