Imaging plasmamembranen Deformations Med pTIRFM

DOI:

10.3791/51334-v

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12:28 min

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April 02, 2014

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Daniel R. Passmore, Tejeshwar C. Rao, Andrew R. Peleman, Arun Anantharam

¹Department of Biological Sciences,Wayne State University

Chapters

00:05Title
01:57pTIRF System Setup
05:54Chromaffin Cell Isolation
07:04pTIRF Image Acquisition
09:14Results: Monitoring Membrane Deformations with pTIRFM
11:58Conclusion

Summary

Automatic Translation

Polarisering-baserede total intern refleksion fluorescensmikroskopi (pTIRFM) muliggør real-time detektering af cellemembran dynamik. Denne artikel beskriver implementeringen af pTIRFM for studiet af membranen remodellering under reguleret exocytose. Teknikken er generaliseres til andre processer i cellebiologi, der direkte eller indirekte involverer ændringer i membran form.

Imaging plasmamembranen Deformations Med pTIRFM

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Chapters

Summary

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