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Journal
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Biology
/
成像质膜变形随着pTIRFM
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Imaging Plasma Membrane Deformations With pTIRFM
Please note that all translations are automatically generated.
Click here for the English version.
成像质膜变形随着pTIRFM
DOI:
10.3791/51334-v
•
12:28 min
•
April 02, 2014
•
Daniel R. Passmore
,
Tejeshwar C. Rao
,
Andrew R. Peleman
,
Arun Anantharam
1
Department of Biological Sciences
,
Wayne State University
Chapters
00:05
Title
01:57
pTIRF System Setup
05:54
Chromaffin Cell Isolation
07:04
pTIRF Image Acquisition
09:14
Results: Monitoring Membrane Deformations with pTIRFM
11:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
基于偏振的全内反射荧光显微镜（pTIRFM）实现实时检测细胞膜动力学。本文介绍pTIRFM的膜重塑的过程中调节胞吐作用的研究落实。该技术推广到在细胞生物学中的其他进程直接或间接地涉及改变膜的形状。
Tags
Keywords: PTIRFM
Membrane Deformation
Exocytosis
Membrane Curvature
Membrane Remodeling
Dense Core Vesicle Fusion
Chromaffin Cells
DiD
Polarization
Evanescent Field
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