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JoVE Journal
Biology

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上皮钠通道(ENaC)的蛋白水解激活通过结合电流测量与检测裂解片段示范
 

上皮钠通道(ENaC)的蛋白水解激活通过结合电流测量与检测裂解片段示范

Article doi: 10.3791/51582
July 5th, 2014 Usage Statistics

Summary July 5th, 2014

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上皮钠通道(ENaC)的异源表达在非洲爪蟾的蛋白水解活化卵母细胞可通过将当前测量与生物素化的方法,调查的离子通道的酶切产物在细胞表面的外观来证明。功能重要的切割位点可通过使用位点定向诱变来识别。

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