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肾单位的组成与功能的成年斑马鱼肾脏分析
JoVE Journal
Biology
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JoVE Journal
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Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney
Please note that all translations are automatically generated.
Click here for the English version.
肾单位的组成与功能的成年斑马鱼肾脏分析
DOI:
10.3791/51644-v
•
08:53 min
•
August 09, 2014
•
Kristen K. McCampbell
,
Kristin N. Springer
,
Rebecca A. Wingert
1
Department of Biological Sciences
,
University of Notre Dame
Chapters
00:05
Title
01:08
Dextran Injection
01:53
Dissection and Mounting in Unfixed Samples
03:13
Fixation and Pigmentation Removal
05:02
Labeling with Alkaline Phosphatase
06:02
DBA Staining
06:49
Results: Proximal Convoluted Tubule (PCT) Visualization
08:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
斑马鱼成人肾脏是一个很好的系统,肾功能恢复及疾病的研究。这种研究的一个重要方面是肾结构和功能的评估。这个协议描述了可被实现来评估肾小管组成,并评估肾重吸收的数种方法。
Tags
Zebrafish
Kidney
Nephron
Renal Regeneration
Neonephrogenesis
Adult Kidney
Kidney Structure
Kidney Function
Genetic Tools
Pharmacological Tools
Kidney Injury
Nephrotoxicant
Cell Ablation
Chronic Disease Modeling
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