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Journal
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Immunology and Infection
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静电粘附实验对整合素激活的T细胞研究
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
Please note that all translations are automatically generated.
Click here for the English version.
静电粘附实验对整合素激活的T细胞研究
DOI:
10.3791/51646-v
•
09:14 min
•
June 13, 2014
•
Marianne Strazza
,
Inbar Azoulay-Alfaguter
,
Ariel Pedoeem
,
Adam Mor
2
1
Department of Medicine
,
New York University School of Medicine
,
2
Departments of Pathology
,
New York University School of Medicine
Chapters
00:05
Title
01:27
Microplate and T Cell Preparation
03:57
Cell Stimulation
05:43
Percent Adherent Cells Analysis
07:11
Results: Representative Stimulated T Cell Data
08:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
静态粘合试验是一种可以用于建模的T淋巴细胞和其他细胞类型之间的相互作用的有力工具。通过标记的T细胞注入到井中涂有粘附分子,而读板器是用于以下串行洗涤量化贴壁细胞的数量所产生的相互作用。
Tags
T Lymphocyte Adhesion
Integrin Activation
LFA-1
ICAM-1
Static Adhesion Assay
Inside-out Signaling
Cell Adhesion
Cell Migration
Immunological Synapse
VLA-4
Fibronectin
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