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Bioengineering
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多步制备技术,以恢复多个代谢化合物的种类进行深入和信息化代谢组学分析
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Please note that all translations are automatically generated.
Click here for the English version.
多步制备技术,以恢复多个代谢化合物的种类进行深入和信息化代谢组学分析
DOI:
10.3791/51670-v
•
11:25 min
•
July 11, 2014
•
Charmion Cruickshank-Quinn
,
Kevin D. Quinn
,
Roger Powell
,
Yanhui Yang
,
Michael Armstrong
,
Spencer Mahaffey
,
Richard Reisdorph
,
Nichole Reisdorph
1
Department of Immunology
,
National Jewish Health
,
2
Department of Pharmacology, School of Medicine
,
University of Colorado Denver
Chapters
00:05
Title
01:22
Protein Precipitation
04:21
Liquid-liquid Extraction
06:55
Solid-phase Extraction
09:12
Results: Metabolites are Effectively Extracted from Biological Fluids by this Protocol
10:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
结果在代谢组学实验的可靠性取决于样品制备的有效性和可重复性。描述的是一个严格和深入的方法,使从生物体液提取的代谢物与随后分析多达数千种化合物中,或感兴趣的只是该化合物类的选项。
Tags
Metabolomics
Sample Preparation
Protein Precipitation
Liquid-liquid Extraction
Solid-phase Extraction
Metabolite Fractionation
Hydrophilic
Hydrophobic
Fatty Acids
Neutral Lipids
Phospholipids
Plasma
Bronchoalveolar Lavage Fluid
Cerebrospinal Fluid
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