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JoVE Journal Environment
Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
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Journal (Environment)
Adherence of Bacteria to Plant Surfaces Measured in the Laboratory
Potentiodynamic Corrosion Testing
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Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Article DOI: 10.3791/51711-v • 13:05 min • June 13th, 2014
June 13th, 2014 •
1, *1, *1, 2, 1
1Institute for Molecular Biotechnology, Bio7, RWTH Aachen University, 2Fraunhofer Institute for Molecular Biology and Applied Ecology
* These authors contributed equally

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Summary
Automatic Translation

Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored using a fluorescent fusion protein, and the protein activity was characterized post-expression.

Tags

Recombinant Cellulase Cel5A Trichoderma Reesei Tobacco Plants Cellulose Degrading Enzymes Plant Expression Methods Large-scale Production Transient Expression Nicotiana Tabacum Fungal Endoglucanase MCherry-enzyme Fusion Protein SDS-PAGE Western Blotting Zymography Substrate Degradation Assays
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