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重组纤维素酶Cel5A从表达<em>里氏木霉</em>在烟草
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Environment
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JoVE Journal
Environment
Expression of Recombinant Cellulase Cel5A from
Trichoderma reesei
in Tobacco Plants
Please note that all translations are automatically generated.
Click here for the English version.
重组纤维素酶Cel5A从表达<em>里氏木霉</em>在烟草
DOI:
10.3791/51711-v
•
13:05 min
•
June 13, 2014
•
Megan Garvey
,
Johannes Klinger*
1
,
Holger Klose*
1
,
Rainer Fischer
,
Ulrich Commandeur
1
Institute for Molecular Biotechnology, Bio7
,
RWTH Aachen University
,
2
Fraunhofer Institute for Molecular Biology and Applied Ecology
Chapters
00:05
Title
01:32
Transient Expression of TrCel5A and TrCel5A-mCherry Proteins
03:17
TrCel5A Extraction from Tobacco Leaves
04:42
SDS-PAGE, Western Blotting, and Azo-CMC Zymography of TrCel5A-mCherry
06:39
4-MUC Activity Assay of TrCel5A
07:50
Azo-carboxymethyl Cellulose Activity Assay of TrCel5A
09:01
PAHBAH Assay of TrCel5A
10:40
Results: Expression of Recombinant Cellulase Cel5A from
Trichoderma reesei
in Tobacco Plants
12:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
烟草植物被用于生产真菌纤维素酶,TrCel5A,通过一个瞬时表达系统。的表达可以用荧光融合蛋白进行监测,并且该蛋白质的活性进行了表征后表达。
Tags
Cellulase
Trichoderma Reesei
Cel5A
Recombinant Expression
Tobacco Plants
Transient Expression
Endoglucanase
SDS-PAGE
Western Blot
Zymography
Substrate Degradation Assay
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