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Medicine
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Fokus Dannelse: En Cell-basert analyse for å bestemme onkogent potensiale av en Gene
JoVE Journal
Medicine
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JoVE Journal
Medicine
Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
Fokus Dannelse: En Cell-basert analyse for å bestemme onkogent potensiale av en Gene
DOI:
10.3791/51742-v
•
08:18 min
•
December 31, 2014
•
Angel Alvarez
,
Gustavo A. Barisone
,
Elva Diaz
1
Department of Pharmacology
,
University of California, Davis
Chapters
00:05
Title
01:17
Retrovirus Production
03:45
NIH 3T3 Cells and Infection
06:02
Staining and Foci Quantification
07:05
Results: Transcription Factor MXD3 as a Gene of Interest (Potential Oncogene)
07:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
Focus Dannelse analysen gir en grei metode for å vurdere trans potensialet for en kandidat onkogen.
Tags
Focus Formation Assay
Oncogenic Potential
Ras Transformation
NIH 3T3 Cells
Retroviral Transduction
Cell Transformation
Contact Inhibition
Anchorage-independent Growth
Tumor Formation
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