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Nanogold Merking av gjær endosomal System for Ultrastructural Analyser
JoVE Journal
Biology
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JoVE Journal Biology
Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
DOI:

09:49 min

July 14, 2014

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Chapters

  • 00:05Title
  • 01:21Spheroplast Preparation
  • 03:07Nanogold Uptake
  • 03:44Fixation and Sectioning
  • 04:42Silver Enhancement for Nanogold Particle Visualization
  • 08:17Results: Yeast Endosomal Compartments are Visualized by Silver-enhanced Nanogold Labeling
  • 09:09Conclusion

Summary

Automatic Translation

Gjær, Saccharomyces cerevisiae, har vært et viktig modellorganisme for å identifisere og studere gener som regulerer biogenesis og funksjoner av endosomal system. Her presenterer vi en detaljert protokoll for den spesifikke merking av endosomal avdelinger for ultra studier.

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