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Immunology and Infection
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在小鼠B淋巴细胞染色体畸变所PNA-FISH快速分析
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
Please note that all translations are automatically generated.
Click here for the English version.
在小鼠B淋巴细胞染色体畸变所PNA-FISH快速分析
DOI:
10.3791/51806-v
•
07:54 min
•
August 19, 2014
•
Sarah M. Misenko
,
Samuel F. Bunting
1
Department of Molecular Biology and Biochemistry
,
Rutgers, the State University of New Jersey
Chapters
00:05
Title
01:02
B Cell Isolation and Activation
03:08
B Cell Fixation, Metaphase Chromosome Spread Preparation, and Telomere PNA FISH
06:10
Results: Chromosome Aberrations in Mouse B Cells as Visualized by PNA Telomere FISH
07:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
DNA修复途径对于维持基因组的完整性,防止基因突变和癌症至关重要。该协议的目的是通过直接观察染色体畸变的量化基因组不稳定性在中期从
原位
杂交(FISH)对端粒DNA重复使用荧光小鼠B细胞的扩散。
Tags
Chromosome Aberrations
Mouse B Lymphocytes
PNA-FISH
DNA Repair
Genomic Instability
Tumorigenesis
Metaphase Chromosome Spreads
Ploidy
Mitotic Index
Primary Cell Population
Transformed Fibroblasts
Patient Cell Lines
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